L-Aspartic Acid

CAS No.:56-84-8

Chemical Name: (S)-Aminosuccinic Acid
Chemical Formula: C4H7NO4
Molecular Weight: 133.1

Specification:
Assay: 98.5%~101.0%
Specific rotation[¦Á]D20: +24¡ã~+26¡ã
pH: 2.5~3.5
Transmittance: ¡Ý98.0%
Loss in drying: ¡Ü0.25%
Residue on ignition: ¡Ü0.10%
Chloride[Cl-]: ¡Ü0.02%
Sulfate[SO42-]: ¡Ü0.02%
Arsenic[As]: ¡Ü1ppm
Heavy metals[Pb]: ¡Ü10ppm
Iron[Fe]: ¡Ü10ppm
Ammonium[NH4+]: ¡Ü0.02%
Other amino acids: Conforms

L-aspartic acid is often used as a component of parenteral and enteral nutrition. As a pharmaceutical ingredient it is used for cell culture and in manufacturing processes. L aspartic acid is widely utilized for mineral supplementation in the salt form. It is produced using an enzymatic reaction.

Reference:
1.Production of L-aspartic acid from Brevibacterium by the cell re-using process.
Terasawa, M | Yukawa, H | Takayama, Y
Process Biochemistry [PROCESS BIOCHEM.]. Vol. 20, no. 4, pp. 124-128. 1985.
Abstract:
Strong aspartase activity was found in cells of Brevibacterium genus, having resistance to alpha -amino-n-butyric acid, and the production of L-aspartic acid from fumaric acid was studied by reusing intact cells. The properties of intracellular aspartase were also investigated. In order to produce the L-aspartic acid at high yield, the conditions which suppressed the unwanted formation of L-malic acid were determined. The production of L-aspartic acid was carried out reusing intact cells which suppressed the side reaction, and L-aspartic acid was produced from fumaric acid stoichiometrically.

2.Basic Studies for Continuous Production of L-Aspartic Acid by Immobilized Escherichia coli Cells
Appl Environ Microbiol. 1974 May; 27(5): 886-889
Abstract:
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg2+ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.