Recombinant Human Epidermal Growth Factor
(EGF)
Name: Recombinant Human Epidermal Growth Factor (rhEGF)
Introduction:
rhEGF is a soluble non carbohydrate protein, which contains 53 amino
acids. The composition, sequence and peptide map of rhEGF are strictly
accord with the natural hEGF. The Amino acid sequence of the first
five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu,
which agrees with the sequence of native human EGF. N-terminal methionine
has been completely removed enzymatically.
Molecular
weight: 6216Da
Characteristics: White powder, PH 6.5-7.5, Isoelectric point 4.6,
acid tolerance(PH=1.0), stable when heated.
Bioactivity:
1.00*106 IU/mg protein Min
Purity:95% Min and 98%(HPLC and SDS-PAGE analysis)Min
Expression
and purification system:
With the application of a high-effective secretive expression system
of Escherichia coli, target protein was secreted directly into the
culture medium after membrane modification. Extremely high bioactivity
achieved due to the remaining of advanced protein structures. By using
full sets of premium separation and purification system, purity of
the production reached above 95%, and the highest can be above 98%.
Application:
rhEGF can promote the treatments of the wounds of skin and cornea,
and has the potential of repairing wounds in alimentary tract, kidney,
liver, lung and trachea. It can be used in the treatments of burns,
all kings of surgical and postoperative wounds and the injury or ulcer
of cornea. Recommended concentration - 0.1-1.0 ng/ml medium depending
on cell type
rhEGF can enhance the growth of new epidermal and stem cells, promote the cell's metabolism and converse differentiation. It is widely used in cosmetics such as whitening, anti-crinkle, crinkle dispelling, anti-aging, etc.
Biological
Activity:
ProSpec's recombinant Human Epidermal Growth Factor is fully biologically
active when compared to standards. The ED50, calculated by the dose-dependant
proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine
uptake) is less then 0.1 ng/ml corresponding to a specific activity
of 1 x 1000000 Units/mg.
Storage:
2-8¡æ(in refrigerator) storage, stable within 36 months.
Endotoxin:
Less than 0.1 ng/¦Ìg (IEU/¦Ìg) of recombinant EGF.
Protein
content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy
at 280 nm using the absorbency value of 2.858 as the extinction coefficient
for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE
computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of human EGF as
a Reference Standard.
Package:
Ultra-pure 98%: 10, 20, 50, 100 g freeze-dried powder
High-pure 95%: 100g-1g freeze-dried powder
Customer's package available
Duration:
Stable for at least three months at 2¡ã to 8¡ãC (lyophilized)
or ¡Ýthree months at -20¡ãC (solubilized). Do not store in frost-free
freezer. Avoid multiple freeze-thaws
Find literature below:
Writen by BARBARA MROCZKOWSKI,' MARTHA REICH,' KATHERINE CHEN,' GRAEME I. BELL,2 AND STANLEY COHEN'
Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennlessee 37232,1 and Howvard Huighes Medical Inistitiute anid Departmenits of Biochemistry, Moleciular Biology, amid Medicine, The University of Chicago, Chicago, Illinois 606372.
Exp Cell Res 2005 Jul 1;307(1):142-52
Invest Ophthalmol Vis Sci 2005 Jun;46(6):2035-41
Anim Reprod Sci 2005 Jul;87(3-4):229-39
Mol Cell Biol 2005 Jun;25(11):4466-75
Cancer Res 2005 May 1;65(9):3877-82
